货品编号:B23317类型:SPRIselect试剂盒规格:5ml商品效期:以产品标识实际效期为准贮藏条件:需18-25℃贮藏Materials•85%Ethanol,non-denatured(ethanolishygroscopic,preparefreshforoptimalresults)•Water(molecularbiologygrade)orastandardbuffersolutionsuchasTris(10mM,pH8)orTE(10mMTris,pH8,1mMEDTA)forDNAelutionSamplePrerequisites•Samplesshouldbefragmenteddouble-strandedDNA。
•SamplesshouldbedissolvedinmolecularbiologygradewaterorstandardbuffersolutionsuchasTrisorTE!SeeEffectsofCommonLaboratoryReagentsonSizeSelectionatendofuserguideformoreinformation。•Samplevolumeshouldbe≥50μL。
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Theguidanceprovidedbelowcanbeusedtooptimizethedesiredsizeselectionrange。UsedmanuallyorautomatedonaliquidhandlingsystemsuchastheBiomekLaboratoryAutomationWorkstation,SPRIselectwillproviderapidandconsistentsizeselectionsuitableformostapplications!
•TomaximizerecoveryforaRightSideSizeSelection,themajorityofthesample’ssizedistributionshouldbesmallerthantheselectionpoint.•TomaximizerecoveryforaDoubleSizeSelection,thesizedistributionshouldbecenteredbetweentheselectionpoints。
Inthisprocess,sizeselectionisrequiredtoproduceauniformdistributionoffragmentsaroundanaeragesize.UsingSPRIselect,thesizedistributioncanbeadjustedtosuittheapplicationandplatformused。Theprocesscanbescaledforlowtohighthroughputworkflows!
AlowervolumewilldecreasepipettingaccuracyofSPRIselect,thereforeincreasingselectionpointvariability!•DNAfragmentsmaybesizeselectedinarangenosmallerthan150bpandnolargerthan800bp.•TomaximizerecoveryforaLeftSideSizeSelection,themajorityofthesample’ssizedistributionshouldbelargerthantheselectionpoint.
江苏B23317使用方法
如果您想了解SPRIselect磁珠法核酸片段筛选试剂贝克曼B23317更多信息,请致电 钊龙:13045610204,或者您直接到我们公司总部一起交流研讨,地址:上海松江区明中路1588弄1072号,我们期待您的致电或来访。
SPRIselec试剂盒基于SPRI的磁珠专利技术,用于NGS核酸特定片段范围筛选.不仅保证了重复性高的实验结果,还可以自由选择需要筛选的片段范围!SPRIselectisaSPRI-basedchemistrythatspeedsandsimplifiesnucleicacidsizeselectionforfragmentlibrarypreparationforNextGenerationsequencing!
热刺一开始选择把主场移到温布利就是因为正在修建的白鹿巷球场无法满足欧冠比赛的要求。但超过85000名球迷在温布利球场看着热刺输给摩纳哥以及勒沃库森后,有多少人还会为热刺对阵莫斯科中央陆军的比赛捧场都还是个未知数。如果热刺能进入欧罗巴联赛的话,继续使用温布利球场,可能会出现球场半空的状况。并且热刺与英足总的协议是欧冠比赛在温布利球场踢,但是其中并没有包含欧罗巴联赛也必须在这里踢的条款。所以热刺换主场的请求也是可以理解的。