- 产品名称:静海核酸筛选试剂盒B23317使用方法_北京核酸筛选试剂盒B23317厂家_上海林钊生物科技有限公司
- 产品价格:面议
- 产品数量:999
- 保质/修期:0
- 保质/修期单位:年
- 更新日期:2023-05-10
•TomaximizerecoveryforaRightSideSizeSelection,themajorityofthesample’ssizedistributionshouldbesmallerthantheselectionpoint!•TomaximizerecoveryforaDoubleSizeSelection,thesizedistributionshouldbecenteredbetweentheselectionpoints.
SPRIselec试剂盒基于SPRI的磁珠专利技术,用于NGS核酸特定片段范围筛选.不仅保证了重复性高的实验结果,还可以自由选择需要筛选的片段范围!SPRIselectisaSPRI-basedchemistrythatspeedsandsimplifiesnucleicacidsizeselectionforfragmentlibrarypreparationforNextGenerationsequencing!
静海核酸筛选试剂盒B23317使用方法
Theguidanceprovidedbelowcanbeusedtooptimizethedesiredsizeselectionrange.UsedmanuallyorautomatedonaliquidhandlingsystemsuchastheBiomekLaboratoryAutomationWorkstation,SPRIselectwillproviderapidandconsistentsizeselectionsuitableformostapplications!
货品编号:B23317类型:SPRIselect试剂盒规格:5ml商品效期:以产品标识实际效期为准贮藏条件:需18-25℃贮藏Materials•85%Ethanol,non-denatured(ethanolishygroscopic,preparefreshforoptimalresults)•Water(molecularbiologygrade)orastandardbuffersolutionsuchasTris(10mM,pH8)orTE(10mMTris,pH8,1mMEDTA)forDNAelutionSamplePrerequisites•Samplesshouldbefragmenteddouble-strandedDNA.
Inthisprocess,sizeselectionisrequiredtoproduceauniformdistributionoffragmentsaroundanaeragesize!UsingSPRIselect,thesizedistributioncanbeadjustedtosuittheapplicationandplatformused!Theprocesscanbescaledforlowtohighthroughputworkflows!
C39291_beckmanC39291代理_上海林钊生物科技有限公司
•SamplesshouldbedissolvedinmolecularbiologygradewaterorstandardbuffersolutionsuchasTrisorTE。SeeEffectsofCommonLaboratoryReagentsonSizeSelectionatendofuserguideformoreinformation。•Samplevolumeshouldbe≥50μL!
AlowervolumewilldecreasepipettingaccuracyofSPRIselect,thereforeincreasingselectionpointvariability!•DNAfragmentsmaybesizeselectedinarangenosmallerthan150bpandnolargerthan800bp!•TomaximizerecoveryforaLeftSideSizeSelection,themajorityofthesample’ssizedistributionshouldbelargerthantheselectionpoint!