•SamplesshouldbedissolvedinmolecularbiologygradewaterorstandardbuffersolutionsuchasTrisorTE!SeeEffectsofCommonLaboratoryReagentsonSizeSelectionatendofuserguideformoreinformation。•Samplevolumeshouldbe≥50μL.
Inthisprocess,sizeselectionisrequiredtoproduceauniformdistributionoffragmentsaroundanaeragesize!UsingSPRIselect,thesizedistributioncanbeadjustedtosuittheapplicationandplatformused。Theprocesscanbescaledforlowtohighthroughputworkflows.
SPRIselect试剂盒B23317报价
货品编号:B23317类型:SPRIselect试剂盒规格:5ml商品效期:以产品标识实际效期为准贮藏条件:需18-25℃贮藏Materials•85%Ethanol,non-denatured(ethanolishygroscopic,preparefreshforoptimalresults)•Water(molecularbiologygrade)orastandardbuffersolutionsuchasTris(10mM,pH8)orTE(10mMTris,pH8,1mMEDTA)forDNAelutionSamplePrerequisites•Samplesshouldbefragmenteddouble-strandedDNA。
上海林钊生物科技有限公司是一家专注区域代理的企业,在SPRIselect磁珠法核酸片段筛选试剂贝克曼B23317领域深耕十几年,对于B23317,有着敏锐的市场嗅觉,丰富的优化经验,扎实的技术团队。秉承互利互惠,合作双赢的理念,坚持客户至上,信誉的原则。致力于从多渠道,多方位,多平台为客户提供的SPRIselect磁珠法核酸片段筛选试剂贝克曼B23317服务,并受到了客户的一致好评。
Theguidanceprovidedbelowcanbeusedtooptimizethedesiredsizeselectionrange.UsedmanuallyorautomatedonaliquidhandlingsystemsuchastheBiomekLaboratoryAutomationWorkstation,SPRIselectwillproviderapidandconsistentsizeselectionsuitableformostapplications.
AlowervolumewilldecreasepipettingaccuracyofSPRIselect,thereforeincreasingselectionpointvariability!•DNAfragmentsmaybesizeselectedinarangenosmallerthan150bpandnolargerthan800bp。•TomaximizerecoveryforaLeftSideSizeSelection,themajorityofthesample’ssizedistributionshouldbelargerthantheselectionpoint。
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SPRIselec试剂盒基于SPRI的磁珠专利技术,用于NGS核酸特定片段范围筛选!不仅保证了重复性高的实验结果,还可以自由选择需要筛选的片段范围!SPRIselectisaSPRI-basedchemistrythatspeedsandsimplifiesnucleicacidsizeselectionforfragmentlibrarypreparationforNextGenerationsequencing!
•TomaximizerecoveryforaRightSideSizeSelection,themajorityofthesample’ssizedistributionshouldbesmallerthantheselectionpoint!•TomaximizerecoveryforaDoubleSizeSelection,thesizedistributionshouldbecenteredbetweentheselectionpoints。
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