AlowervolumewilldecreasepipettingaccuracyofSPRIselect,thereforeincreasingselectionpointvariability.•DNAfragmentsmaybesizeselectedinarangenosmallerthan150bpandnolargerthan800bp.•TomaximizerecoveryforaLeftSideSizeSelection,themajorityofthesample’ssizedistributionshouldbelargerthantheselectionpoint!

　　Theguidanceprovidedbelowcanbeusedtooptimizethedesiredsizeselectionrange.UsedmanuallyorautomatedonaliquidhandlingsystemsuchastheBiomekLaboratoryAutomationWorkstation,SPRIselectwillproviderapidandconsistentsizeselectionsuitableformostapplications！

　　SPRIselec试剂盒基于SPRI的磁珠专利技术，用于NGS核酸特定片段范围筛选.不仅保证了重复性高的实验结果，还可以自由选择需要筛选的片段范围。SPRIselectisaSPRI-basedchemistrythatspeedsandsimplifiesnucleicacidsizeselectionforfragmentlibrarypreparationforNextGenerationsequencing!

原装进口B23317核酸片段筛选试剂盒

　　•TomaximizerecoveryforaRightSideSizeSelection,themajorityofthesample’ssizedistributionshouldbesmallerthantheselectionpoint。•TomaximizerecoveryforaDoubleSizeSelection,thesizedistributionshouldbecenteredbetweentheselectionpoints.

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　　•SamplesshouldbedissolvedinmolecularbiologygradewaterorstandardbuffersolutionsuchasTrisorTE.SeeEffectsofCommonLaboratoryReagentsonSizeSelectionatendofuserguideformoreinformation。•Samplevolumeshouldbe≥50μL。

　　货品编号：B23317类型：SPRIselect试剂盒规格：5ml商品效期：以产品标识实际效期为准贮藏条件：需18-25℃贮藏Materials•85%Ethanol,non-denatured(ethanolishygroscopic,preparefreshforoptimalresults)•Water(molecularbiologygrade)orastandardbuffersolutionsuchasTris(10mM,pH8)orTE(10mMTris,pH8,1mMEDTA)forDNAelutionSamplePrerequisites•Samplesshouldbefragmenteddouble-strandedDNA!

　　Inthisprocess,sizeselectionisrequiredtoproduceauniformdistributionoffragmentsaroundanaeragesize。UsingSPRIselect,thesizedistributioncanbeadjustedtosuittheapplicationandplatformused。Theprocesscanbescaledforlowtohighthroughputworkflows！